Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5396-5400.doi: 10.3969/j.issn. 2095-4344.2012.29. 016

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Comparison of cytotoxicity among three gingival retraction cords on human gingival fibroblasts in vitro

Ni Ying1, Zhang Xiao-ming2, Hui Min1, Liu Jian3   

  1. 1College of Stomatology, Binzhou Medical University, Binzhou 256603, Shandong Province, China;
    2Department of Prosthodontics, the Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China;
    3Department of Orthodontics, Rizhao Hospital of Traditional Chinese Medicine, Rizhao 276800, Shandong Province, China
  • Received:2011-12-29 Revised:2012-01-20 Online:2012-07-15 Published:2012-07-15
  • Contact: Zhang Xiao-ming, Master, Associate professor, Department of Prosthodontics, the Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China
  • About author:Ni Ying★, Studying for master’s degree, College of Stomatology, Binzhou Medical University, Binzhou 256603, Shandong Province, China niying860711@ 163.com

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Abstract:

BACKGROUND: In recent years, the gingival retraction effect of gingival retraction drugs is a hot topic for domestic and foreign scholars, while the biocompatibility of the commercial gingival retraction cord is less involved.
OBJECTIVE: To evaluate the cytotoxicity effect of three kinds of gingival retraction cords on human gingival fibroblasts, and to provide theoretical basis for choosing proper gingival retraction cords in clinic.
METHODS: Three kinds of gingival retraction cords (epinephrine-impregnated cord, aluminium sulphate-impregnated cord and non-drug-impregnated cord) were incubated in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum for 24 hours, according to 1:1 and 1:4 dilution, and then the cords acted on gingival fibroblasts cultured in vitro. Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum was used as negative control group.
RESULTS AND CONCLUSION: The results of MTT assay demonstrated that the three kinds of gingival retraction cords all could inhibit human gingival fibroblasts growth and proliferation. The cytotoxicity of the three kinds of gingival retraction cords in the leaching liquor with 1:1 dilution was significantly higher than that of 1:4 dilution and control groups (P < 0.05). The cytotoxicity of epinephrine-impregnated cord in the leaching liquor with 1:4 dilution was significantly higher than that of the control group (P < 0.05). The results of transmission electron microscope showed that some cells in the epinephrine-impregnated cord group were found apoptosis. These findings suggest that the three kinds of gingival retraction cords all have certain cytotoxicity to gingival fibroblasts. The cytotoxicity of epinephrine-impregnated cord is the biggest, followed by aluminium sulphate-impregnated cord, and the cytotoxicity of the non-drug-impregnated cord is the smallest.

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